An antibody test for the virus that causes COVID-19, developed by researchers at The University of Texas at Austin in collaboration with Houston Methodist and other institutions, is more accurate and can handle a much larger number of donor samples at lower overall cost than standard antibody tests currently in use. In the near term, the test can be used to accurately identify the best donors for convalescent plasma therapy and measure how well candidate vaccines and other therapies elicit an immune response.
Additional uses coming later that are likely to have the biggest societal impact, the researchers say, are to assess relative immunity in those previously infected by the SARS-CoV-2 virus and identify asymptomatic individuals with high levels of neutralizing antibodies against the virus.
The UT Austin research team, led by Jason Lavinder, a research associate in the Cockrell School of Engineering, and Greg Ippolito, assistant professor in the College of Natural Sciences and Dell Medical School, developed the new antibody test for SARS-CoV-2 and provided the viral antigens for this study via their UT Austin colleague and collaborator, associate professor Jason McLellan. Other UT Austin team members are Dalton Towers and Jimmy Gollihar. The work was published this week in The Journal of Clinical Investigation.
“This is potentially game-changing when it comes to serological testing for COVID-19 immunity,” Lavinder said. “We can now use highly scalable, automated testing to examine antibody-based immunity to COVID-19 for hundreds of donors in a single run. With increased levels of automation, limited capacity for serological testing can be rapidly addressed using this approach.”
The gold standard of COVID-19 antibody testing measures the amount of virus neutralizing (VN) antibodies circulating in the blood, because this closely correlates with immunity. However, this kind of antibody testing is not widely available because it’s technically complex; requires days to set up, run and interpret; and needs to be performed in a biosafety level 3 laboratory.
The research team, therefore, looked to another type of test, called ELISA assays, that can be implemented and performed with relative ease in a high-throughput fashion and are widely available and extensively used in clinical labs across the world. The ELISA tests, or enzyme-linked immunosorbent assays, look at whether antibodies against specific SARS-CoV-2 proteins are present and produce a quantitative measure of those antibodies.